tag:blogger.com,1999:blog-22150296515637817042024-03-13T10:50:33.195-07:00Hughes-Fulford Lab Millie Hughes-Fulfordhttp://www.blogger.com/profile/02674379357521084975noreply@blogger.comBlogger37125tag:blogger.com,1999:blog-2215029651563781704.post-59619297689627010102015-03-13T12:03:00.001-07:002015-03-13T12:04:48.496-07:00It's Finally Science Time!After we got our samples back from Long Beach, CA we have been busy making sure everything is ready to go for our samples! They are, after all, very important. We first collected all of our samples from the EUs that were still in their ECs. Since we've been back, we have kept them in the -80C freezer, waiting for the chance to thaw them and collect the contents. That in and of itself was a long and tedious process for 40 samples. It took the better part of a work day to finish; but, it was still very exciting knowing that these samples went to space and back and could potentially teach us many things. Once everything was collected, we returned the hardware back to KI.<br />
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<tr><td style="text-align: center;"><a href="http://2.bp.blogspot.com/-Fgdx_6nf5JI/VQMx-0h1DCI/AAAAAAAAAKc/BU_ciMnZkt4/s1600/IMG_3028.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://2.bp.blogspot.com/-Fgdx_6nf5JI/VQMx-0h1DCI/AAAAAAAAAKc/BU_ciMnZkt4/s1600/IMG_3028.JPG" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Pier Luigi and Fabio from KI with Francis from Nasa Ames with the returned hardware</td></tr>
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Now that we have all our precious samples, we were now able to start the exciting part! First, we needed to extract the RNA from the samples. We have extracted about half our samples which already took a few days.<br />
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<tr><td class="tr-caption" style="text-align: center;">Tara in the process of extracting our samples from space. </td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Tara using the NanoDrop to measure the sample's concentration, purity, and among other things </td></tr>
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Now we are waiting on the next step which is to send a portion of the samples off to another facility to see the differences in genes expressed in a 1g environment and ug environment. Christina Limhttp://www.blogger.com/profile/12661249643767950673noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-56221595276095729272015-02-17T16:31:00.000-08:002015-02-27T23:35:35.345-08:00T Cells are Back from SpaceThis past week, Tara, Emily, and I traveled down to Long Beach to retrieve our samples. During our trip we got to visit the SpaceX facility in Hawthorne, CA. They were definitely doing very innovative things and we were more than excited to be able to take a look!<br />
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<tr><td class="tr-caption" style="text-align: center;">T-Cell Team at SpaceX Facility in Hawthorne, CA</td></tr>
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Later that night, our samples arrived to a private air strip in the Long Beach Airport. Overall it was a very smooth hand over! We were first to get our samples off the back of the hollowed out plane and we carefully made sure we got every single EU of our precious samples. All the EUs stayed in the dry ice as we made our way back up to San Francisco with some very special cargo. <br />
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<tr><td class="tr-caption" style="text-align: center;">T-Cell Team inside the plane before we got our samples</td></tr>
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<tr><td style="text-align: center;"><a href="http://3.bp.blogspot.com/-_GesOq4M9Zc/VPELb5i16yI/AAAAAAAAAJM/Z8HhtIEL6xg/s1600/IMG_2823.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://3.bp.blogspot.com/-_GesOq4M9Zc/VPELb5i16yI/AAAAAAAAAJM/Z8HhtIEL6xg/s1600/IMG_2823.JPG" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Tara is especially excited to have received our precious samples</td></tr>
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<br />Christina Limhttp://www.blogger.com/profile/12661249643767950673noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-73977108128798182822015-01-19T22:38:00.004-08:002015-01-19T22:38:42.434-08:00Ground Controls and MoreAfter the launch, Emily and I were definitely excited to fill our ground controls. We then celebrated with a great breakfast and had a chance to rest after such an eventful day. For the next couple days, we packed up the lab and got a chance to finally explore Kennedy Space Center and everything it had to offer.<br />
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<tr><td class="tr-caption" style="text-align: center;">Boxes upon boxes to ship back to the lab</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Must-have meatball picture at the KSC Visitor Center</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Astronaut Hall of Fame: what a great hidden treasure just a few miles outside of KSC</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">The Saturn V Rocket was enormous! </td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Our last day at Residence Inn</td></tr>
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Lastly, we got to see the on-orbit operations and perform the ground controls with a two-hour delay. <br />
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<tr><td class="tr-caption" style="text-align: center;">Early morning view of on-orbit operations. </td></tr>
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<tr><td class="tr-caption" style="text-align: center;">All suited up for ground controls. </td></tr>
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<tr><td style="text-align: center;"><a href="http://2.bp.blogspot.com/-WMTYVU3j55g/VL3yH-UnvCI/AAAAAAAAAHs/xMC_7FMLWso/s1600/IMG_0222.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://2.bp.blogspot.com/-WMTYVU3j55g/VL3yH-UnvCI/AAAAAAAAAHs/xMC_7FMLWso/s1600/IMG_0222.JPG" height="239" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">We had a special chance to visit the Orion!</td></tr>
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With that, it was finally time to head to Orlando and fly back to San Francisco. We had a great time at Kennedy Space Center and we will definitely miss it.<br />
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Christina Limhttp://www.blogger.com/profile/12661249643767950673noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-16968142972664353782015-01-11T20:19:00.001-08:002015-01-11T20:19:22.846-08:00SpaceX CRS-5 HAS LAUNCHED <table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
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<tr><td class="tr-caption" style="text-align: center;">SpaceX CRS-5 Launch </td></tr>
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SpaceX's Dragon with the T-Cell Experiment onboard has finally launched at 4:47am EST! Words cannot even begin to describe the emotions as we saw the rocket go up into the sky. Emily, BJ, Karin, and I stood on the causeway anxiously awaiting, as the announcer counted 3...2...1...LIFT OFF! The rocket lit up the dark, early morning sky into nothing we have ever seen before. As we watched the bright shining light soar into the heavens and beyond, the sight alone was all worth the long days and nights working on this project. This was such an amazing experience that we are so privileged to be a part of. <br /><br />Even with a successful launch, our trip is not over yet. There are still many things to take care of in the next few days. But for now, there's definitely time for a well deserved rest. Christina Limhttp://www.blogger.com/profile/12661249643767950673noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-17130137900120402352015-01-09T17:42:00.002-08:002015-01-09T17:42:23.104-08:00L-8h (again) It's been a whirlwind of activity the last few days. Our first launch attempt on Tuesday (it seems so long ago now) was scrubbed just under 2 minutes before the scheduled launch. We were all disappointed, of course, and returned to the lab that evening to re-do our prep and wait for confirmation on the next launch date.<br />
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Thankfully, it wasn't pushed too far back and is now set for tomorrow morning at 4:47AM. Christina and I had another long day of T-cell isolation yesterday and are both looking forward to getting up early tomorrow morning to see the Falcon off. Go T-cell!<br />
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<tr><td style="text-align: center;"><a href="http://2.bp.blogspot.com/-_9p5-_grv4s/VLCDL2Oq7RI/AAAAAAAAAXY/OINTNWiURWs/s1600/ScienceTeam.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://2.bp.blogspot.com/-_9p5-_grv4s/VLCDL2Oq7RI/AAAAAAAAAXY/OINTNWiURWs/s1600/ScienceTeam.JPG" height="240" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">T-cell preparations, take two</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Filled EUs and EU tools. These are going to space tomorrow!</td></tr>
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Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-25305585123220546062015-01-05T20:47:00.000-08:002015-01-05T20:48:22.780-08:00L-7 hoursIt's been an exhausting couple days, but we have done everything we can! We have isolated the T-Cells from the blood of donors, filled the experimental units, and handed them over. Emily and I with the help of BJ and Karin were able to work through the long hours it all took. It's such a relief to be done for now! Here are some pictures to document the process: <br />
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<tr><td style="text-align: center;"><a href="http://2.bp.blogspot.com/-n7p-ekyFOhI/VKtm3W4wWZI/AAAAAAAAAFY/70I3dpeo_-0/s1600/IMG_1799.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://2.bp.blogspot.com/-n7p-ekyFOhI/VKtm3W4wWZI/AAAAAAAAAFY/70I3dpeo_-0/s1600/IMG_1799.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">KI and T-Cell Team after the handover of EUs and tools</td></tr>
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<tr><td style="text-align: center;"><a href="http://2.bp.blogspot.com/-2pJPqngzA3U/VKtm1pmLMsI/AAAAAAAAAFA/YQU1VUa0UI8/s1600/IMG_2634.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://2.bp.blogspot.com/-2pJPqngzA3U/VKtm1pmLMsI/AAAAAAAAAFA/YQU1VUa0UI8/s1600/IMG_2634.JPG" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Emily and Christina isolating the T-Cells</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Filled EUs ready to be transported. </td></tr>
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Emily and I were invited to visit the SpaceX rocket that was ready for launch. Our samples are on the capsule! It was such a great and gratifying experience to see the rocket in person. <br />
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<tr><td style="text-align: center;"><a href="http://3.bp.blogspot.com/-ebQJ26JFseU/VKtnTM8x0YI/AAAAAAAAAFg/Q7AhAHe82hs/s1600/IMG_0153.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://3.bp.blogspot.com/-ebQJ26JFseU/VKtnTM8x0YI/AAAAAAAAAFg/Q7AhAHe82hs/s1600/IMG_0153.jpg" height="400" width="298" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">SpaceX Rocket ready to launch</td></tr>
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<tr><td style="text-align: center;"><a href="http://3.bp.blogspot.com/--3dL-xUgYJY/VKtm2K2wW2I/AAAAAAAAAFI/6k4WzOX28u8/s1600/IMG_0163.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://3.bp.blogspot.com/--3dL-xUgYJY/VKtm2K2wW2I/AAAAAAAAAFI/6k4WzOX28u8/s1600/IMG_0163.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Obligatory selfie by the rocket. </td></tr>
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And now we wait, for the final few hours before the launch!Christina Limhttp://www.blogger.com/profile/12661249643767950673noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-82300914572738137732015-01-01T19:58:00.001-08:002015-01-01T20:01:30.067-08:00Back at CapeL-5! Christina and I arrived in Cape Canaveral from SFO this evening, refreshed from the winter holidays and looking forward to the launch attempt on January 6th. We'll be getting our samples tomorrow evening and will start our preparation for our T-cell isolation on Saturday. Everything for launch is still a go, so we're all very hopeful this is the real deal.Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-57710561352657679792014-12-18T20:28:00.000-08:002014-12-18T20:28:10.593-08:00SpaceX-CRS 5 Mission is POSTPONED
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After being at NASA Kennedy Space Center for a little over a
week, the mission has been postponed until January 6, 2015. You can read more <a href="http://spaceflightnow.com/2014/12/18/launch-of-spacex-supply-ship-delayed-to-january/" target="_blank">here</a>. We were a little disappointed, but definitely
happy to be home for Christmas. We’ll be
back soon!<br /><o:p></o:p></div>
<!--EndFragment-->Christina Limhttp://www.blogger.com/profile/12661249643767950673noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-34891047567674299102014-12-15T15:21:00.000-08:002014-12-17T05:52:35.109-08:00First Week for SpaceX-CRS5 Mission<!--[if gte mso 9]><xml>
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arrived in Orlando, Florida after a full day of travel from San Francisco. We couldn’t have asked for a better day to leave, especially since we missed the massive storm that hit the Bay Area
shortly after we left. Since then, we’ve
had some time to relax and prepare ourselves for the mission launch scheduled
for Friday, December 19<sup>th</sup>. </span><!--EndFragment--><br />
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<span style="font-family: Cambria; font-size: 12.0pt; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: "MS 明朝"; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin;">Here are some pictures of the things we've been able to do:</span><br />
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<tr><td class="tr-caption" style="text-align: center;">Selfie in front of NASA Kennedy Space Center Head Quarters</td></tr>
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<tr><td style="text-align: center;"><a href="http://4.bp.blogspot.com/-u5C1H-dgDOQ/VI9o5zfHOjI/AAAAAAAAAC8/hJRKA-UAYIA/s1600/11083_10153028254832275_6333767782420149145_n.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://4.bp.blogspot.com/-u5C1H-dgDOQ/VI9o5zfHOjI/AAAAAAAAAC8/hJRKA-UAYIA/s1600/11083_10153028254832275_6333767782420149145_n.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Obligatory picture in front of Residence Inn's Astronaut</td></tr>
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<tr><td style="text-align: center;"><a href="http://3.bp.blogspot.com/-k_nStXZsJb0/VI9o66BbHJI/AAAAAAAAADE/t2fyauL8TTQ/s1600/IMG_1635.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://3.bp.blogspot.com/-k_nStXZsJb0/VI9o66BbHJI/AAAAAAAAADE/t2fyauL8TTQ/s1600/IMG_1635.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Dr. Jeffrey Smith, NASA Ames Research Center's Space Biology Project Manager, took a tour of our T-Cell Lab </td></tr>
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<tr><td style="text-align: center;"><a href="http://1.bp.blogspot.com/-7pkF5SYe6q4/VI9o7JAWiLI/AAAAAAAAADM/ddHY6oPORNk/s1600/IMG_1641.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://1.bp.blogspot.com/-7pkF5SYe6q4/VI9o7JAWiLI/AAAAAAAAADM/ddHY6oPORNk/s1600/IMG_1641.jpg" height="300" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">The tour continued to the ESA Hardware Lab where the Experimental Units are processed and kept</td></tr>
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<tr><td style="text-align: center;"><a href="http://3.bp.blogspot.com/-YmUGuXckSrE/VI9o6GuNIxI/AAAAAAAAAC4/1yf-oR0UZRA/s1600/IMG_1623.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://3.bp.blogspot.com/-YmUGuXckSrE/VI9o6GuNIxI/AAAAAAAAAC4/1yf-oR0UZRA/s1600/IMG_1623.JPG" height="400" width="300" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">We visited Cold Stowage to see where our samples will be held after we finish processing them</td></tr>
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<span style="font-family: Cambria; font-size: 12.0pt; mso-ansi-language: EN-US; mso-ascii-theme-font: minor-latin; mso-bidi-font-family: "Times New Roman"; mso-bidi-language: AR-SA; mso-bidi-theme-font: minor-bidi; mso-fareast-font-family: "MS 明朝"; mso-fareast-language: EN-US; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin;"><br /></span>Christina Limhttp://www.blogger.com/profile/12661249643767950673noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-30481454410904377782014-11-05T13:09:00.000-08:002014-11-06T09:31:17.219-08:00My First Month at the Hughes-Fulford Lab<!--[if gte mso 9]><xml>
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<tr><td class="tr-caption" style="text-align: center;">Field Trip to the Warehouse With Our First Shipment</td></tr>
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Today marks my first month here at the Hughes-Fulford
Lab.<span style="mso-spacerun: yes;"> </span>Time has definitely gone by faster
than I ever imagined; but more importantly, I have learned so much in just my
first month here.<span style="mso-spacerun: yes;"> </span>I came to my first day
of work just a couple days after relocating from Los Angeles.<span style="mso-spacerun: yes;"> </span>Everything was new: the culture, the
weather, and just everything that I needed to learn before our trip to Cape
Canaveral in December.<span style="mso-spacerun: yes;"> </span><span style="mso-spacerun: yes;"></span>Learning everything has
definitely been hectic for all of us.<span style="mso-spacerun: yes;">
</span>But luckily, Emily and Tara are the best for helping me through my
countless questions, and training me so that everything becomes second
nature.<span style="mso-spacerun: yes;"> </span>Even though I’m sure I have more
to learn, hopefully after our SpaceX 5 launch, things will be a little
less hectic.<span style="mso-spacerun: yes;"> </span>But until then, lots to
pack and even more to learn! I’m definitely looking forward to the time I have here at this lab.<span style="mso-spacerun: yes;"> </span></div>
Christina Limhttp://www.blogger.com/profile/12661249643767950673noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-52105902173860496292014-10-23T15:10:00.001-07:002014-10-23T15:13:39.037-07:00Packing again...It's almost that time again...<br />
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<a href="http://4.bp.blogspot.com/-VT1-YfSxD7w/VEl72U9x6bI/AAAAAAAAAUA/j8E9r9NDueA/s1600/SpaceX5%2Blaunch%2B(NET).png" imageanchor="1" style="clear: left; float: left; margin-bottom: 1em; margin-right: 1em;"><img border="0" src="http://4.bp.blogspot.com/-VT1-YfSxD7w/VEl72U9x6bI/AAAAAAAAAUA/j8E9r9NDueA/s1600/SpaceX5%2Blaunch%2B(NET).png" height="125" width="530" /></a></div>
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Our next experiment on SpaceX CRS-5 is just around the corner. We're welcoming a new team member, Christina, aboard for the upcoming mission.<br />
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We've also started to gather all our supplies for shipping. We've made some pretty impressive piles!<br />
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<a href="http://4.bp.blogspot.com/-nQQ5g7hTYpc/VEl8fLrBXcI/AAAAAAAAAUI/N7ZrAUgsO6E/s1600/photo%2B2.JPG" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="http://4.bp.blogspot.com/-nQQ5g7hTYpc/VEl8fLrBXcI/AAAAAAAAAUI/N7ZrAUgsO6E/s1600/photo%2B2.JPG" height="320" width="239" /></a></div>
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<br />Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-69304765002072271322014-07-08T12:05:00.000-07:002014-07-08T12:26:59.926-07:00Science timeNow that we have our samples back on Earth, it is time to get to the real work!<br />
<br /><a href="http://2.bp.blogspot.com/-rjXejROLb_s/U7wtPXikgLI/AAAAAAAAAJU/xbWgMWN2Gek/s1600/photo6.jpg" imageanchor="1" style="clear: left; float: left; margin-bottom: 1em; margin-right: 1em;"><img border="0" src="http://2.bp.blogspot.com/-rjXejROLb_s/U7wtPXikgLI/AAAAAAAAAJU/xbWgMWN2Gek/s1600/photo6.jpg" height="240" width="320" /></a>The samples were first thawed and RNA extracted from the T-cells. This step took several days alone to complete. We then ran the RNA on chips in the Agilent Bioanalyzer to determine quality and concentration.<br />
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We were all more than a little jittery during this part - imagine messing up and destroying the RNA at this stage of the experiment (AFTER it had flown to and returned from space?!)! Luckily, we had no problems and are now excited to get to the analysis part of the project :).<br />
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The next step will be performed by the UCSF Gladstone facility - they will run the RNA on GeneChips that will tell us which genes are expressed in the microgravity samples versus the 1g control samples.<br />
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<tr><td style="text-align: center;"><a href="http://3.bp.blogspot.com/-JrXXV2TvIFQ/U7wtPBNBMyI/AAAAAAAAAJQ/BZzjHVObKV8/s1600/photo5.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://3.bp.blogspot.com/-JrXXV2TvIFQ/U7wtPBNBMyI/AAAAAAAAAJQ/BZzjHVObKV8/s1600/photo5.jpg" height="292" width="320" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Tara in the process of isolating RNA</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Agilent Bioanalyzer machine</td></tr>
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<br />Miya Yoshidahttp://www.blogger.com/profile/03070978020218217380noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-67541324139478827812014-05-22T13:34:00.000-07:002014-07-08T12:08:37.315-07:00T-cells from space!We are all now back in San Francisco after a successful retrieval of our samples from the Dragon capsule!<br />
<a href="http://3.bp.blogspot.com/-yY0YCEuyxDM/U3-zvGnQWxI/AAAAAAAAAHg/aRWld4vwcss/s1600/photo+5.JPG" imageanchor="1" style="clear: left; float: left; margin-bottom: 1em; margin-right: 1em;"><img border="0" src="http://3.bp.blogspot.com/-yY0YCEuyxDM/U3-zvGnQWxI/AAAAAAAAAHg/aRWld4vwcss/s1600/photo+5.JPG" height="240" width="320" /></a>After Dragon splashed down on May 18, the boat sent to pick it up encounted very high sea levels. We didn't get our samples back until 5pm on May 20. NASA called us to a private airstrip right next to the Long Beach airport, where we waited for all the payloads and cargo to be brought from the dock. As many of the items were continuing on to Johnson Space Center in Houston, everything was loaded onto the airplane before it could be retrieved. <br />
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<tr><td class="tr-caption" style="text-align: center;">Millie next to her mission summary, STS-40!<br />
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Millie, Emily, and I then boarded the plane, put our samples onto dry ice, and set off for San Francisco. <br />
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This morning, we disassembled all the hardware and collected our samples. The hardware will be going back with Kayser Italia (who flew into SF) to Italy until SpaceX-5 preparation begins.<br />
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We were also able to view the SpaceX factory (unfortunately no pictures allowed) and the Endeavour shuttle exhibit while in LA.<br />
<a href="http://3.bp.blogspot.com/-DHdpe2fftKc/U3-zrtEOXKI/AAAAAAAAAHM/5-boPaE-cvo/s1600/photo+2.JPG" imageanchor="1" style="clear: left; float: left; margin-bottom: 1em; margin-right: 1em;"> </a><a href="http://3.bp.blogspot.com/-DHdpe2fftKc/U3-zrtEOXKI/AAAAAAAAAHM/5-boPaE-cvo/s1600/photo+2.JPG" imageanchor="1" style="clear: left; float: left; margin-bottom: 1em; margin-right: 1em;"> </a><br />
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<tr><td style="text-align: center;"><a href="http://1.bp.blogspot.com/-2AP6gDSnpcQ/U3-0r8HCBnI/AAAAAAAAAIE/0rZVCdgPa7k/s1600/photo+5.JPG" imageanchor="1" style="clear: left; margin-bottom: 1em; margin-left: auto; margin-right: auto;"><img border="0" src="http://1.bp.blogspot.com/-2AP6gDSnpcQ/U3-0r8HCBnI/AAAAAAAAAIE/0rZVCdgPa7k/s1600/photo+5.JPG" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Boarding the charter jet</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Thank you Cold Stowage!</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">SpaceX factory in Hawthorne</td></tr>
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Miya Yoshidahttp://www.blogger.com/profile/03070978020218217380noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-78209713272873642362014-05-14T15:58:00.000-07:002014-05-14T16:00:27.855-07:00Splashdown - Baja, CA<div class="separator" style="clear: both; text-align: center;">
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The Hughes-Fulford Lab is now headed to Long Beach, CA!<br />
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Splashdown is projected to occur on May 18. It is approximately 5.5 hours from undocking to splashdown. A boat will go out to sea to pick up the Dragon capsule, where all the samples and payloads will be removed and stored at the appropriate temperatures. The T-cell Activation in Aging samples will be stored in a -80C freezer. The boat then takes a couple of days to return to shore and all samples are transferred to the Long Beach airport. We will pick up our samples directly from the aircraft as many of the other payloads will be flying on to Johnson Space Center in Houston.<br />
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We'll bring the samples (still in the Experiment Units) back to our lab in San Francisco. Together with Kayser Italia, who is flying in from Italy, we will disassemble all the hardware and collect the samples in tubes to freeze back. KI will be taking the hardware back to their lab in Italy to prepare for SpaceX-5. Miya Yoshidahttp://www.blogger.com/profile/03070978020218217380noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-68095613878800426682014-04-21T18:00:00.000-07:002014-05-14T16:00:56.306-07:00Dragon DockingSpaceX has some beautiful pictures of the Dragon capsule before and after berthing yesterday. Enjoy!<br />
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<br />Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-37603210154924231962014-04-21T12:58:00.000-07:002014-05-14T15:43:36.235-07:00Ground Controls to Major Rick<br />
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<tr><td style="text-align: center;"><a href="http://www.nasa.gov/sites/default/files/exp39_042114_med.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="1" src="http://www.nasa.gov/sites/default/files/exp39_042114_med.jpg" height="240" width="320" /></a></td></tr>
<tr><td 320="" class="tr-caption" style="text-align: center;">T-cell in space! Astronaut Rick Mastracchio loads our cassettes into the KUBIK centrifuge (photo: NASA)</td></tr>
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We've made it to the ISS! Or at least our samples have. The Dragon capsule berthing was complete this morning, and T-cell was one of the first payloads unloaded. The cold bag containing our experimental cassettes was handed off to astronaut Rick Mastracchio, who performed the on-board operations. While it would be very neat to have been up there with the samples, we settled (happily) for watching a live stream from the ISS. <br />
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<tr><td class="tr-caption" style="text-align: center;">All dressed up to do our ground controls.</td></tr>
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Speaking of our samples, we had a pretty busy day in lab doing our ground controls. In addition to the 10 experimental containers sent to the ISS, we filled four additional cassettes on our long isolation day to stay with us on the ground. This morning, we moved them to a special room that mimics the conditions on-board the ISS. In full clean-room outfitting - hairnet, gloves, lab coats and booties - we then incubated, activated and fixed our ground samples just like our samples on board (with a two hour delay so we could get the exact time between each step right).<br />
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On one of our many plane trips between SF and Florida the last few
weeks
(they all blend together now) we watched the Secret Life of Walter
Mitty, which featured a version of David Bowie's "Space Oddity." It's
been stuck in our head this past week, and seemed very fitting for this post. <br />
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It's crazy to be done with this phase of the
experiment -- we've been working towards this for more than a year! Now
all we have to do is wait to get our samples back for analysis and the
real science (and fun) will begin. <br />
<br />Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-11367933426528700302014-04-19T15:02:00.000-07:002014-05-14T15:24:20.501-07:00More Pictures of LaunchHere are more (and closer) pictures of the launch from the <a href="http://mediaarchive.ksc.nasa.gov/" rel="nofollow" target="_blank">KSC media archive</a>:<br />
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<a href="http://www-pao.ksc.nasa.gov/images/medium/2014-2177-m.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="http://www-pao.ksc.nasa.gov/images/medium/2014-2177-m.jpg" height="400" width="265" /></a><a href="http://www-pao.ksc.nasa.gov/images/medium/2014-2156-m.jpg" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="http://www-pao.ksc.nasa.gov/images/medium/2014-2156-m.jpg" height="265" width="400" /></a></div>
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<br />Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-35553609051609596862014-04-18T20:00:00.001-07:002014-04-18T20:08:36.508-07:00Up, up, and away!<div class="separator" style="clear: both; text-align: center;">
<a href="http://1.bp.blogspot.com/-cmHEpzE7wYo/U1Hjt02nSnI/AAAAAAAAAOA/SrxyFIsEC-k/s1600/photo+2+(3).JPG" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="http://1.bp.blogspot.com/-cmHEpzE7wYo/U1Hjt02nSnI/AAAAAAAAAOA/SrxyFIsEC-k/s1600/photo+2+(3).JPG" height="320" width="239" /></a></div>
T-Cell has taken off as of 3:25 EDT this afternoon! We are excited, relieved, and waiting in anticipation for berthing with the ISS. It's been a long journey, with delays, a scrub, two all-nighters, and it was beyond words to watch the bright streak of the rocket rise up across the grey sky and disappear into the heavens above. <br />
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We have a few days to wait for T-Cell opps onboard. Until then, we wish safe travels to the Dragon capsule on it's way to the ISS. And our gratitude to all the support on the ground - from our lab support at KSC to cold stowage to all the teams working like crazy this last week to make the launch possible. Hurrah to SpaceX for a flawless launch! Go Dragon!Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-79295538852070889222014-04-14T14:52:00.000-07:002014-04-21T13:27:32.981-07:00Charlie Bolden, Head of NASA<table align="center" cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
<tr><td style="text-align: center;"><a href="http://2.bp.blogspot.com/-PDO8cYC2S5U/U02p1mQGNHI/AAAAAAAAAFE/A-ZM9likf4Q/s1600/IMG_1214.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://2.bp.blogspot.com/-PDO8cYC2S5U/U02p1mQGNHI/AAAAAAAAAFE/A-ZM9likf4Q/s1600/IMG_1214.JPG" height="225" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">SpaceX Falcon 9 on the launch pad</td></tr>
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<tr><td style="text-align: center;"><a href="http://4.bp.blogspot.com/-kus4UcUPDWo/U02pf6dB7tI/AAAAAAAAAE8/MW3WXDbxa68/s1600/Bolden+and+team.JPG" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://4.bp.blogspot.com/-kus4UcUPDWo/U02pf6dB7tI/AAAAAAAAAE8/MW3WXDbxa68/s1600/Bolden+and+team.JPG" height="225" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Charlie Bolden, BJ Navarro, Emily, Millie, Tara, Miya</td></tr>
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We attended the SpaceX mission briefing and had a chance to meet Charlie Bolden, the current administrator of NASA! He and Millie are old friends.<br />
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Miya Yoshidahttp://www.blogger.com/profile/03070978020218217380noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-61479057778525557382014-04-14T13:03:00.000-07:002014-04-21T13:06:55.793-07:00LC 40Today we, along with the other payloads and guests, were invited to view the Falcon 9 rocket vertical on the launch pad! We got pretty close on the grass just outside the pad - it was EXTREMELY bright outside..<br />
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<a href="http://2.bp.blogspot.com/-UgVHkPAZnYM/U1V6Aizh6gI/AAAAAAAAAFY/wjZFXBAP2cQ/s1600/IMG_0021.JPG" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="http://2.bp.blogspot.com/-UgVHkPAZnYM/U1V6Aizh6gI/AAAAAAAAAFY/wjZFXBAP2cQ/s1600/IMG_0021.JPG" height="300" width="400" /></a></div>
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<a href="http://3.bp.blogspot.com/-5RTK1rT_m-I/U1V6LTb8r8I/AAAAAAAAAFo/jZp6UURNMjw/s1600/Science+Team+at+Launch+Site.JPG" imageanchor="1" style="margin-left: 1em; margin-right: 1em;"><img border="0" src="http://3.bp.blogspot.com/-5RTK1rT_m-I/U1V6LTb8r8I/AAAAAAAAAFo/jZp6UURNMjw/s1600/Science+Team+at+Launch+Site.JPG" height="400" width="400" /></a></div>
<br />Miya Yoshidahttp://www.blogger.com/profile/03070978020218217380noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-81073302335056941992014-04-12T23:51:00.004-07:002014-04-21T18:04:05.334-07:00The Night Shift<table cellpadding="0" cellspacing="0" class="tr-caption-container" style="margin-left: auto; margin-right: auto; text-align: center;"><tbody>
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<tr><td class="tr-caption" style="text-align: center;"><b>SSPF as we entered tonight</b></td></tr>
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The Space Station Processing Facility (SSPF) is the location of our lab.<span style="mso-spacerun: yes;"> </span>It is midnight on 4/13/2014 and Tara, Miya,
Emily and I have been here since 9:30 PM EST along with our NASA support teams.<span style="mso-spacerun: yes;"> </span><o:p></o:p></div>
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<tr><td class="tr-caption" style="text-align: center;"><b>KI hands over hardware to T Cell Team</b></td></tr>
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Handover of the flight hardware from Kayser Italia to the T Cell Team in the laboratory before the start of isolation of the T cells.<o:p></o:p></div>
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<tr><td style="text-align: center;"><a href="http://4.bp.blogspot.com/-agoK_WCsdN4/U0oxLM0FdpI/AAAAAAAAATs/LP2nzl8FT0w/s1600/LRS+blood+collection.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://4.bp.blogspot.com/-agoK_WCsdN4/U0oxLM0FdpI/AAAAAAAAATs/LP2nzl8FT0w/s1600/LRS+blood+collection.jpg" height="320" width="240" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;"><b>LRS sitting in a 50 ml tube</b></td></tr>
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It will be a long night since we are isolating T-cells from
the LRS and buffy coat blood bank samples.<span style="mso-spacerun: yes;">
</span>We have 10 independent human samples prepared; we use the best 8 for the
experiment (n=8). The photo on the left shows a LRS at the start of
isolation.<span style="mso-spacerun: yes;"> </span>Isolation is finished around
3 AM; we then start loading the samples into flight hardware.<span style="mso-spacerun: yes;"> </span></div>
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<b><span style="font-size: x-small;">Experimental Unit holds four independent donor samples</span></b></div>
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Each experiment unit takes approximately 45 minutes to fill
and we will finish approximately at 10AM EST. <span style="mso-spacerun: yes;"> </span>That is when Kayser Italia (our ESA hardware maker) begin the leak testing the experiment units. The experimental unit is shown to the right, each cell is filled with T cells from individual donors (n=4) we have two EUs for each condition so the total number of donors is 8 for each gravity condition.</div>
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<b>More Pictures of the team:</b><br />
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<tr><td style="text-align: center;"><a href="http://3.bp.blogspot.com/-um6vmgKq_Qw/U1V7kcAgikI/AAAAAAAAAUY/fIyLIahorq0/s1600/20140412_224018.jpg" imageanchor="1" style="margin-left: auto; margin-right: auto;"><img border="0" src="http://3.bp.blogspot.com/-um6vmgKq_Qw/U1V7kcAgikI/AAAAAAAAAUY/fIyLIahorq0/s1600/20140412_224018.jpg" height="225" width="400" /></a></td></tr>
<tr><td class="tr-caption" style="text-align: center;">Starting to process our blood samples</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Filling the Experimental Units (EUs)</td></tr>
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Millie Hughes-Fulfordhttp://www.blogger.com/profile/02674379357521084975noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-78351267002114703862014-03-31T17:56:00.003-07:002014-03-31T17:57:46.203-07:00There and back againUnfortunately, we're back in San Francisco again after a week in the Cape. The launch slated for last night was canceled due to range issues -- you can read more on the Spaceflight Now website <a href="http://spaceflightnow.com/news/n1403/30range/" target="_blank"><span style="color: blue;">here</span></a>. We're still waiting for confirmation on the next launch date, but we're hopeful that we'll get T-cell up soon!Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-89587997670463051222014-03-17T14:08:00.002-07:002014-03-17T14:09:34.457-07:00L-13<br />
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The SpaceX3 launch has been delayed until March 30th, so the countdown clock is back to L-13. The team is back in San Francisco briefly, all a little disappointed that the experiment didn't go up on the 16th. We took some selfies to cheer ourselves up, and are still very excited for our next opportunity!<br />
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<tr><td class="tr-caption" style="text-align: center;">Nice shirts!</td></tr>
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<tr><td class="tr-caption" style="text-align: center;">Full science team photo. Go T-Cell!</td></tr>
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<br />Anonymoushttp://www.blogger.com/profile/12992864901818121461noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-51269250832728349032014-03-13T08:17:00.003-07:002014-03-13T08:20:18.675-07:00T-cell Team<div class="separator" style="clear: both; text-align: center;">
More photographs of our lab here at KSC and our team!</div>
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Tara, Miya, Emily, and Millie:</div>
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Science team and Kayser Italia</div>
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Us plus the NASA folks from both KSC and ARC:</div>
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Tomorrow will be our big day of T-cell isolation before the launch. We plan to start at 8am and will likely work until 2am. Fingers crossed that the rocket launches on March 16!</div>
<br />Miya Yoshidahttp://www.blogger.com/profile/03070978020218217380noreply@blogger.comtag:blogger.com,1999:blog-2215029651563781704.post-44258538902573210062014-03-10T08:13:00.000-07:002014-03-13T08:14:42.585-07:00First day<div class="separator" style="clear: both; text-align: center;">
We're at KSC! Photographers have come by to take pictures of Millie and the lab. We headed over to the Kayser Italia (ESA) lab quickly to take a look at the flight hardware and equipment.</div>
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Miya Yoshidahttp://www.blogger.com/profile/03070978020218217380noreply@blogger.com